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51.
Hydrobiologia - In regulated rivers, dams alter longitudinal gradients in flow regimes, geomorphology, water quality and temperature with associated impacts on aquatic biota. Unregulated... 相似文献
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Julie C Robidart Christina M Preston Ryan W Paerl Kendra A Turk Annika C Mosier Christopher A Francis Christopher A Scholin Jonathan P Zehr 《The ISME journal》2012,6(3):513-523
Monterey Bay, CA is an Eastern boundary upwelling system that is nitrogen limited much of the year. In order to resolve population dynamics of microorganisms important for nutrient cycling in this region, we deployed the Environmental Sample Processor with quantitative PCR assays targeting both ribosomal RNA genes and functional genes for subclades of cyanobacteria (Synechococcus) and ammonia-oxidizing Archaea (Thaumarchaeota) populations. Results showed a strong correlation between Thaumarchaea abundances and nitrate during the spring upwelling but not the fall sampling period. In relatively stratified fall waters, the Thaumarchaeota community reached higher numbers than in the spring, and an unexpected positive correlation with chlorophyll concentration was observed. Further, we detected drops in Synechococcus abundance that occurred on short (that is, daily) time scales. Upwelling intensity and blooms of eukaryotic phytoplankton strongly influenced Synechococcus distributions in the spring and fall, revealing what appear to be the environmental limitations of Synechococcus populations in this region. Each of these findings has implications for Monterey Bay biogeochemistry. High-resolution sampling provides a better-resolved framework within which to observe changes in the plankton community. We conclude that controls on these ecosystems change on smaller scales than are routinely assessed, and that more predictable trends will be uncovered if they are evaluated within seasonal (monthly), rather than on annual or interannual scales. 相似文献
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The Coastal Wetlands Management Project (CWMP), funded by the Global Environment Facility and implemented by the Ghana Wildlife Department, seeks to preserve the ecological integrity of coastal lagoons that serve as important sites for migratory waterbirds. This report describes the geomorphology, hydrology, soils, water chemistry, and vegetation of the Muni-Pomadze Ramsar site. Muni lagoon is a saline, shallow water lagoon separated from the sea by a sand bar which may be breached occasionally. The bulk of the rainfall in the catchment evaporates or flows as surface runoff into three streams that empty into Muni lagoon. Open water in the lagoon varies seasonally from 100 ha in the dry season to over 1000 ha in the wet season. The natural flora of the site can be divided into four main types; flood plain (including mangrove and wetland vegetation), dune vegetation, riverine vegetation, and terrestrial vegetation on elevated ground. The latter consists of a combination of grasslands, thickets, and Eucalyptus plantations. Fifty-three percent of the site is classified as natural vegetation. An additional 32.5% is agricultural land and 12.6% is residential area for the 11 communities within the site. The main source of employment are farming or fishing. The area surrounding Muni lagoon is used extensively for bushmeat hunting and as the tribal hunting grounds of the Efutu people. Apart from the global importance of the Muni-Pomadze site for biodiversity, management of the site is further justified by its considerable potential for development as an income-generating and educational nature reserve with an eco-cultural theme, managed by the local communities in partnership with the governmental and non-governmental conservation agencies. 相似文献
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Ravinder Raju Bernard F. Castillo Stewart K. Richardson Meena Thakur Ryan Severins Mitchell Kronenberg Amy R. Howell 《Bioorganic & medicinal chemistry letters》2009,19(15):4122-4125
Four 3″- and 4″-deoxy and -fluorogalactosyl ceramides were synthesized, and their ability to stimulate iNKT cells, based on levels of IL-2 production, was assessed in three NKT cell receptor hybridomas. In two of the hybridomas, 1.2 and 2H4, all of the analogs were immunostimulatory, while in the 1.4 hybridoma only the 4″-fluoro analog led to the production of significant levels of IL-2. 相似文献
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Silas A. Buck Thomas Steinkellner Despoina Aslanoglou Michael Villeneuve Sai H. Bhatte Victoria C. Childers Sophie A. Rubin Briana R. De Miranda Emma I. O'Leary Elizabeth G. Neureiter Keri J. Fogle Michael J. Palladino Ryan W. Logan Jill R. Glausier Kenneth N. Fish David A. Lewis J. Timothy Greenamyre Brian D. McCabe Claire E. J. Cheetham Thomas S. Hnasko Zachary Freyberg 《Aging cell》2021,20(5)
Age is the greatest risk factor for Parkinson''s disease (PD) which causes progressive loss of dopamine (DA) neurons, with males at greater risk than females. Intriguingly, some DA neurons are more resilient to degeneration than others. Increasing evidence suggests that vesicular glutamate transporter (VGLUT) expression in DA neurons plays a role in this selective vulnerability. We investigated the role of DA neuron VGLUT in sex‐ and age‐related differences in DA neuron vulnerability using the genetically tractable Drosophila model. We found sex differences in age‐related DA neurodegeneration and its associated locomotor behavior, where males exhibit significantly greater decreases in both DA neuron number and locomotion during aging compared with females. We discovered that dynamic changes in DA neuron VGLUT expression mediate these age‐ and sex‐related differences, as a potential compensatory mechanism for diminished DA neurotransmission during aging. Importantly, female Drosophila possess higher levels of VGLUT expression in DA neurons compared with males, and this finding is conserved across flies, rodents, and humans. Moreover, we showed that diminishing VGLUT expression in DA neurons eliminates females'' greater resilience to DA neuron loss across aging. This offers a new mechanism for sex differences in selective DA neuron vulnerability to age‐related DA neurodegeneration. Finally, in mice, we showed that the ability of DA neurons to achieve optimal control over VGLUT expression is essential for DA neuron survival. These findings lay the groundwork for the manipulation of DA neuron VGLUT expression as a novel therapeutic strategy to boost DA neuron resilience to age‐ and PD‐related neurodegeneration. 相似文献
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Petronela Weisová Ujval Anilkumar Caitriona Ryan Caoimhín G. Concannon Jochen H.M. Prehn Manus W. Ward 《BBA》2012,1817(5):744-753
The preconditioning response conferred by a mild uncoupling of the mitochondrial membrane potential (Δψm) has been attributed to altered reactive oxygen species (ROS) production and mitochondrial Ca2 + uptake within the cells. Here we have explored if altered cellular energetics in response to a mild mitochondrial uncoupling stimulus may also contribute to the protection. The addition of 100 nM FCCP for 30 min to cerebellar granule neurons (CGNs) induced a transient depolarization of the Δψm, that was sufficient to significantly reduce CGN vulnerability to the excitotoxic stimulus, glutamate. On investigation, the mild mitochondrial ‘uncoupling’ stimulus resulted in a significant increase in the plasma membrane levels of the glucose transporter isoform 3, with a hyperpolarisation of Δψm and increased cellular ATP levels also evident following the washout of FCCP. Furthermore, the phosphorylation state of AMP-activated protein kinase (AMPK) (Thr 172) was increased within 5 min of the uncoupling stimulus and elevated up to 1 h after washout. Significantly, the physiological changes and protection evident after the mild uncoupling stimulus were lost in CGNs when AMPK activity was inhibited. This study identifies an additional mechanism through which protection is mediated upon mild mitochondrial uncoupling: it implicates increased AMPK signalling and an adaptive shift in energy metabolism as mediators of the preconditioning response associated with FCCP-induced mild mitochondrial uncoupling. 相似文献